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Hur antibody

WebWe determined that inhibitors of HuR (ELAVL1), a prosurvival mRNA stability factor that regulates cyclin D1, and an inhibitor of Yes … WebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # MA1-167. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) and RNA Immunoprecipitation …

Innate immune responses through Toll-like receptor 3 require …

WebAll lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml Lane 1 : Wild-type SW480 cell lysate Lane 2 : ELAVL1 knockout SW480 cell lysate Lane 3 : HEK-293 cell lysate Lane 4 … WebHuR inhibitor KH-3 is a small molecule, selective inhibitior of HuR function with Ki of 0.83 and 0.72 uM in FP and AlphaLISA assays, respectively. KH-3 potently inhibits breast cancer cell growth in vitro and in vivo. KH-3 inhibits breast cancer cell invasion in vitro as well as delays initiation of lung colonies and improves mouse survival in ... pottery barn dillon https://ppsrepair.com

HuR Polyclonal Antibody (11910-1-AP) - thermofisher.com

WebUsing a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas. WebApplications. This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. RIP lysate from MCF7 cells (2 X 10 6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-HuR antibody. WebHUR Antibody Purified Rabbit Polyclonal Antibody (Pab) Anti-HUR Antibody at 1:1000 dilution + Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat … toughcrete

Identification of a target RNA motif for RNA-binding protein HuR

Category:Hu proteins regulate alternative splicing by inducing localized

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Hur antibody

HuR antibody (11910-1-AP) Proteintech - ptglab

Web13 aug. 2024 · Embryonic lethal abnormal vision-like 1/human antigen R (ELAVL/HuR), a canonical 3′ untranslated region AU-rich element-binding protein, is a crucial mRNA-stabilizing protein that protects target mRNAs from RNA-destabilizing protein- or microRNA-induced silencing complex (miRISC)-mediated degradation. Web"HuR antibodies" comparison. At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with …

Hur antibody

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Web22 dec. 2014 · The mRNA binding protein HuR is over expressed in cancer cells and contributes to disease progression through post-transcriptional regulation of mRNA. The regulation of HuR and how this relates to glioma is the focus of this report. SRC and c-Abl kinases regulate HuR sub-cellular trafficking and influence accumulation in the … Web21 mrt. 2024 · HUR 3 4 5 ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) 2 3 Embryonic Lethal, Abnormal Vision, Drosophila, Homolog-Like 1 2 3 …

WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and … WebHuR Antibody (3A2) is an IgG 1 κ mouse monoclonal HuR antibody (also designated ELAV like RNA binding protein 4 antibody or ELAVL4 antibody) suitable for the detection of the HuR protein of mouse, rat, human and Xenopus laevis origin by WB, IP, IF, IHC(P) and ELISA. HuR Antibody (3A2) is available as both the non-conjugated anti-HuR …

Web25 However, although HuR acts as a positive regulator, CUGBP1 acts as a negative regulator of TJ/AJ proteins by competing with HuR for binding to the 3 0 UTR of occludin and E-cadherin. 22 In our ... Web1 dag geleden · The following primary antibodies were used for immunoblotting: antibodies against HA Tag (3724, CST), HuR (2582, CST), and pERK1/2 (4370, CST) were used at 1:1,000 dilution, and antibodies against Vinculin (13901, CST) and Tubulin (T6199, Sigma) were used at 1:3,000 dilution as the internal controls.

WebHerein, we have extended our recently reported findings on the ability of HuR to associate with the immunopurified from mammalian cell extracts hnRNP and mRNP complexes by the application of an anti-HuR antibody that selects HuR-RNP complexes.

Web1 aug. 2011 · Anti-HuR antibody (Left) and anti-HuB antibody (Right) were used. U1 70 K was used as a loading control. β-tubulin III is a neuronal marker. ( E ) CoIP was carried out with nonspecific IgG or antibodies against hypophosphorylated RNAPII (8WG16), ser-2 phosphorylated RNAPII (H5), or ser-5 phosphorylated RNAPII (H14) using protein lysate … pottery barn diaper bag reviewWebCompare anti-SERPINB13 Antibody ABIN1398419 from antibodies-online on Biocompare.com. Welcome Guest. Sign In Register. Products. Popular ... NCBI Gene Aliases headpin, HUR7, HU, 5430417G24, HURPIN, HSHUR7SEQ, HUR, PI13, PI, hea; Clonality Polyclonal; Research Area Proteolysis / Ubiquitin; Add to Compare List. Local … tough cover hydraulic hoseWebDescription. HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. pottery barn diffuser fragranceWebAntibodies that detect HuR can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ELISA. … tough crewWeb23 feb. 2004 · Proteins of the Hu family of RNA-binding proteins, which comprises the ubiquitous HuR and the primarily neuronal proteins HuB, HuC, and HuD (1-5), are emerging as pivotal regulators of posttranscriptional gene expression.Hu proteins possess three RNA-recognition motifs through which they bind with high affinity and specificity to target labile … tough cover lawn tractor covertoughcrete stainWebImmunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody (ab196626) Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626 at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel). Western blot - Anti-HuR / ELAVL1 antibody (ab196626) pottery barn diffuser oil